To be eligible for B-CAST a study/ invasive breast cancer cases must participate in BCAC, and have ethical approval that includes targeted DNA sequencing of germline and tumour DNA. Studies/cases should also have core data available and genotyping data from iCOGS, OncoArray or GWAS.
B-CAST includes two tissue-based components with the following sample requirements:
- Slides from Tissue microarrays (TMA) for centralised staining/scoring a panel of 15 IHC/ISH.
- Tumour samples (FFPE or fresh frozen) and paired germline (blood) DNA for centralised DNA extraction and targeted sequencing.