One of the goals of the B-CAST project is DNA extraction and targeted sequencing of ~10,000 tumour samples (WP3). The first objective was to develop a panel for the targeted sequencing. Diether Lambrechts and colleagues (VIB, KULeuven) evaluated online data sources for somatic mutations in breast cancer and developed a final panel of 107 genes. Please find below an overview of the selected genes. In addition to these genes, homozygous deleted and amplified genes will be added as well as hotspot promotor regions.